C.8.12.6 Whole Slide Microscopy Functional Group Macros

The following sections contain Functional Group macros specific to the VL Whole Slide Microscopy Image IOD.

Note: The attribute descriptions in the Functional Group Macros are written as if they were applicable to a single frame (i.e., the macro is part of the Per-frame Functional Groups Sequence). If an attribute is applicable to all frames (i.e. the macro is part of the Shared Functional Groups Sequence) the phrase “this frame” in the attribute description shall be interpreted to mean “for all frames”.

C.8.12.6.1 Plane Position (Slide) Macro

Table C.8.12.6.1-1 specifies the attributes of the Plane Position (Slide) Functional Group macro.

Table C.8.12.6.1-1PLANE POSITION (SLIDE) MACRO ATTRIBUTES

Attribute Name Tag Type Attribute Description
Plane Position (Slide) Sequence (0048,021A) 1 Describes position of frame in the Total Pixel Matrix and in the Slide Coordinate System Frame of Reference. Only a single Item shall be included in this Sequence.
>Column Position In Total Pixel Matrix (0048,021E) 1 The column position of the top left hand pixel of the frame in the Total Pixel Matrix (see C.8.12.4.1.1). The column position of the top left pixel of the Total Pixel Matrix is 1.
>Row Position In Total Pixel Matrix (0048,021F) 1 The row position of the top left hand pixel of the frame in the Total Pixel Matrix (see C.8.12.4.1.1). The row position of the top left pixel of the Total Pixel Matrix is 1.
>X Offset in Slide Coordinate System (0040,072A) 1 The X offset in mm from the Origin of the Slide Coordinate System. See Figure C.8-16.
>Y Offset in Slide Coordinate System (0040,073A) 1 The Y offset in mm from the Origin of the Slide Coordinate System. See Figure C.8-16.
>Z Offset in Slide Coordinate System (0040,074A) 1 The Z offset in μm from the Origin of the Slide Coordinate System, nominally the surface of the glass slide substrate. See Figure C.8-17 Note: Required even if only a single focal plane was acquired.

C.8.12.6.2 Optical Path Identification Macro

Table C.8.12.6.2-1 specifies the attributes of the Optical Path Identification Functional Group macro.

Table C.8.12.6.2-1OPTICAL PATH IDENTIFICATION MACRO ATTRIBUTES

Attribute Name Tag Type Attribute Description
Optical Path Identification Sequence (0048,0207) 1 Identifies the optical path characteristics of this frame. Only a single Item shall be included in this sequence.
>Optical Path Identifier (0048,0106) 1 Uniquely identifies the path described in the Optical Path Sequence (0048,0105) by reference to an Item with the same Optical Path Identifier (0048,0106) value. See C.8.12.5.

C.8.12.6.3 Specimen Reference Macro

Table C.8.12.6.3-1 specifies the attributes of the Specimen Reference Functional Group macro. This allows association of the frame with specific specimens imaged on the same slide (e.g., tissue micro-arrays).

Table C.8.12.6.3-1SPECIMEN REFERENCE MACRO ATTRIBUTES

Attribute Name Tag Type Attribute Description
Specimen Reference Sequence (0048,0110) 2 Identifies specimens that may be visible in this frame, and which are fully identified in the Specimen Description Sequence (0040,0560). Zero or more Items shall be included in this sequence.
>Specimen UID (0040,0554) 1 Unique Identifier for Specimen. See Section C.7.6.22.1.2.