A.32.8 VL Whole Slide Microscopy Image Information Object Definition

A.32.8.1 VL Whole Slide Microscopy Image IOD Description

The VL Whole Slide Microscopy Image IOD specifies the Attributes of a multi-frame visible light whole slide microscopy image encoded as a tiled decomposition. Each frame encodes a single tile within a three-dimensional imaged volume at a uniform resolution.

Notes: 1. An entire set of tiles for an acquisition may be encoded in the frames of a single SOP Instance, in multiple SOP Instances of a single concatenation, or in multiple SOP Instances in a series (with or without concatenations). E.g., a single SOP Instance may contain an entire low resolution image as a single tile (single frame), or a single SOP Instance may contain an entire high resolution, multi-focal depth, multi-spectral acquisition (multiple frames).

2. Attention is called to the possibility that conformance with the VL Whole Slide Microscopy Image Storage SOP Class utilizing this IOD may involve the use of claimed Intellectual Property Rights, among which may be US Patent 6,272,235 referenced in Section 2. The DICOM Standards Committee takes no position concerning the evidence, validity or applicability of claimed Intellectual Property Rights, whether asserted by members of the DICOM Standards Committee or others.

A.32.8.2 VL Whole Slide Microscopy IOD Entity-Relationship Model

The E-R Model in section A.1.2 depicts those components of the DICOM Information Model that comprise the VL Whole Slide Microscopy IOD.

A.32.8.3 VL Whole Slide Microscopy IOD Module Table

Table A.32.8-1VL WHOLE SLIDE MICROSCOPY IOD MODULES

IE Module Reference Usage
Patient Patient C.7.1.1 M
Clinical Trial Subject C.7.1.3 U
Study General Study C.7.2.1 M
Patient Study C.7.2.2 U
Clinical Trial Study C.7.2.3 U
Series General Series C.7.3.1 M
Whole Slide Microscopy Series C.8.12.3 M
Clinical Trial Series C.7.3.2 U
Frame of Reference Frame of Reference C.7.4.1 M
Equipment General Equipment C.7.5.1 M
Enhanced General Equipment C.7.5.2 M
Image General Image C.7.6.1 M
Image Pixel C.7.6.3 M
Acquisition Context C.7.6.14 M
Multi-frame Functional Groups C.7.6.16 M
Multi-frame Dimension C.7.6.17 M
Specimen C.7.6.22 M
Whole Slide Microscopy Image C.8.12.4 M
Optical Path C.8.12.5 M
Multi-Resolution Navigation C.8.12.7 C – Required if Image Type (0008,0008) Value 3 is LOCALIZER
Slide Label C.8.12.8 C – Required if Image Type (0008,0008) Value 3 is LABEL; may be present otherwise
ICC Profile C.11.15 U
SOP Common C.12.1 M
Common Instance Reference C.12.2 M
Frame Extraction C.12.3 C - Required if the SOP Instance was created in response to a Frame-Level retrieve request

A.32.8.3.1 VL Whole Slide Microscopy IOD Content Constraints

A.32.8.3.1.1 Optical Path Module

The Code Sequences within the Optical Path Sequence (0048,0105) of the Optical Path Module (see C.8.12.5) are constrained as follows:

Baseline Context ID for Illuminator Type Code Sequence (0048,0100) is CID 8125.

Baseline Context ID for Illumination Color Code Sequence (0048,0108) is CID 8122.

Baseline Context ID for Illumination Type Code Sequence (0022,0016) is CID 8123.

Baseline Context ID for Lenses Code Sequence (0022,0019) is CID 8121.

Baseline Context ID for Light Path Filter Type Stack Code Sequence (0022,0017) and for Image Path Filter Type Stack Code Sequence (0022,0018) is CID 8124.

Baseline Context ID for Channel Description Code Sequence (0022,001A) is CID 8122.

A.32.8.4 VL WHOLE SLIDE MICROSCOPY FUNCTIONAL GROUP MACROS

Table A.32.8-2 specifies the use of the Functional Group macros used in the Multi-frame Functional Groups Module for the VL Whole Slide Microscopy IOD.

Table A.32.8-2VL WHOLE SLIDE MICROSCOPY FUNCTIONAL GROUP MACROS

Functional Group Macro Section Usage
Pixel Measures C.7.6.16.2.1 M – Shall be used as a Shared Functional Group.
Frame Content C.7.6.16.2.2 M – Shall not be used as a Shared Functional Group.
Referenced Image C.7.6.16.2.5 U
Derivation Image C.7.6.16.2.6 C – Required if the image or frame has been derived from another SOP Instance.
Real World Value Mapping C.7.6.16.2.11 U
Plane Position (Slide) C.8.12.6.1 M
Optical Path Identification C.8.12.6.2 M
Specimen Reference C.8.12.6.3 U

A.32.8.4.1 VL Whole Slide Microscopy Functional Group Macros Content Constraints

A.32.8.4.1.1 Referenced Image

Baseline Context ID for Purpose of Reference Code Sequence (0040,A170) is CID 8120.

A.32.8.4.1.2 Plane Position (Slide)

Frames shall occupy plane positions in a regular tiling of the Total Image Matrix; the frames may extend beyond the edges of the Total Image Matrix. Therefore, the value of Column Position In Total Pixel Matrix (0048,021E) shall be an integer multiple of the value of Columns (0028,0011) plus a constant, and the value of Row Position In Total Pixel Matrix (0048,021F) shall be an integer multiple of the value of Rows (0028,0010) plus a (possibly different) constant.

Note: Pixels beyond the edge of the Total Image Matrix may be encoded with a fixed padding value. For images with MONOCHROME2 Photometric Interpretation, see Pixel Padding Value (0028,0120) in Section C.7.5.1.1.2.

The encoding of tiles may be sparse; i.e., some tiles may not be encoded in frames. There are no constraints on the ordering of frames within the pixel data; each frame specifies its position in the Plane Position (Slide) Functional Group.

Note: Different SOP Instances within a Series may have different tiling boundaries and sizes.

Frames associated with different optical paths may coincide in any spatial dimension.